CST, 27907S, GAPDH (14C10) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3007)

Monoclonal Antibody for studying GAPDH. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, GAPDH (14C10) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3007) (CST #27907) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity GAPDH (14C10) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3007) detects endogenous levels of total GAPDH protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys260 of human GAPDH protein. Background Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5). Alternate Names aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; MGC88685; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; Peptidyl-cysteine S-nitrosylase GAPDH Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG