CST, 27913SF, Calponin 1 (D8L2T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying calponin 1. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, Calponin 1 (D8L2T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #27913) is ready to ship. Product Usage Information This product is the carrier free version of product #17819. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 17819 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Calponin 1 (D8L2T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Calponin 1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly234 of human Calponin 1 protein. Background Calponin 1 is a member of the Calponin family, which consists of Calponins 1, 2, and 3. Calponin 1 is exquisitely expressed in smooth muscle cells of various tissues, where it interacts with filamentous F-actin to regulate smooth muscle contraction (1). Alternate Names Basic calponin; calponin 1; calponin 1, basic, smooth muscle; Calponin H1, smooth muscle; Calponin-1; calponins, basic; CNN1; epididymis secretory protein Li 14; HEL-S-14; Sm-Calp; SMCC Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG