CST, 27960S, LIMP-2/SCARB2 (E2Z5F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SCARB2 mouse. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, LIMP-2/SCARB2 (E2Z5F) Rabbit Monoclonal Antibody (CST #27960) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 69463 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity LIMP-2/SCARB2 (E2Z5F) Rabbit Monoclonal Antibody recognizes endogenous levels of total LIMP-2/SCARB2 protein. LIMP-2/SCARB2 is glycosylated at multiple sites, and it migrates at 80 kDa and 54 kDa in its glycosylated and unglycosylated states, respectively. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly467 of mouse LIMP-2/SCARB2 protein. Background Lysosomal integral membrane protein type 2 (LIMP-2, also known as SCARB2) is a lysosomal membrane glucoprotein encoded by the gene. As a lysosome-enriched protein, LIMP-2/SCARB2 contributes to endosomal and lysosomal transport and function in the cell. β-glucocerebrosidase (GBA), a lysosomal enzyme defective in Gaucher disease and genetically linked to Parkinson's disease, is targeted to lysosomes by binding directly to LIMP-2/SCARB2 via a mannose 6-phosphate-independent pathway (1-3). LIMP-2/SCARB2 may also play a role in the transport of cholesterol from the lysosome to the membrane, an important function as cholesterol contributes to various biophysical properties of cell membranes and may contribute to various diseases (4,5). Interestingly, SCARB2 is upregulated in neurodegenerative diseases, including Lewy body disease (6), and genetic variants in the gene encoding SCARB2 are linked to Parkinson's disease (7), suggesting that altered lysosomal function may contribute to neuropathology in these diseases. LIMP-2/SCARB2 also serves as a receptor for enterovirus 71 and coxsackieviruses (8). Alternate Names 85 kDa lysosomal membrane sialoglycoprotein; 9330185J12Rik; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2; Cd36l; Cd36l2; LGP; LGP85; LIM; LIMP II; LIMP-2; Lysosome membrane protein 2; Lysosome membrane protein II; MLGP85; Scarb2; Scavenger receptor class B member 2; scavenger receptor class B, member 2; SCRB2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 54, 80 Source/Isotype: Rabbit IgG