CST, 27964T, Syntenin-1/MDA9 (E2I9L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying syntenin. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Syntenin-1/MDA9 (E2I9L) Rabbit Monoclonal Antibody (CST #27964) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 32485 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Syntenin-1/MDA9 (E2I9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total syntenin-1/MDA9 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human syntenin-1/MDA9 protein. Background Syntenin-1, also known as MDA9 or SDCBP, is a member of the PDZ family of proteins (1), defined by the presence of a PDZ protein interaction domain. Syntenin-1, via its PDZ domain, functions as a scaffold adaptor protein that regulates numerous signal transduction pathways, including FAK, Src, TGF-βR1, EGFR, and IGF1R (2-5). Aberrantly elevated expression of syntenin-1 has been found in various cancers and has been linked to the growth and invasion of tumor cells (5-7). In the central nervous system, syntenin-1 has been shown to mediate neuron dendrite protrusion and spine formation through its interaction with syndecan and Rheb (8). Syntenin-1 is also a key component of the syndecan/syntenin/ALIX pathway, and it has been shown to contribute directly to the formation and internalization of exosomes (9-11). Alternate Names MDA-9; MDA9; melanoma differentiation associated protein-9; Melanoma differentiation-associated protein 9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; Scaffold protein Pbp1; SDCB1; SDCBP; ST1; SYCL; syndecan binding protein; syndecan binding protein (syntenin); Syndecan-binding protein 1; syntenin; Syntenin-1; TACIP18 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG