CST, 2796S, Lyn (C13F9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Lyn. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Lyn (C13F9) Rabbit Monoclonal Antibody (CST #2796) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 48103 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Lyn (C13F9) Rabbit Monoclonal Antibody detects endogenous levels of total Lyn protein. This antibody does not cross-react with any other Src-family members. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human Lyn. Background Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8). Alternate Names FLJ26625; JTK8; Lck/Yes-related novel protein tyrosine kinase; LYN; LYN proto-oncogene, Src family tyrosine kinase; p53Lyn; p56Lyn; Tyrosine-protein kinase Lyn; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Rabbit IgG