CST, 27987T, Adora2b/Adenosine Receptor A2b (E8V7W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ADORA2B. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Adora2b/Adenosine Receptor A2b (E8V7W) Rabbit Monoclonal Antibody (CST #27987) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Adora2b/Adenosine Receptor A2b (E8V7W) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse Adora2b/Adenosine Receptor A2b protein. This antibody cross-reacts with human Adenosine receptor A2a protein but not with mouse Adenosine receptor A2a protein or human Adenosine receptor A2b protein. This antibody detects a 140 kDa band of unknown origin, and in human cell lines, detects a 42 kDa band of unknown origin. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse Adora2b/Adenosine Receptor A2b protein. Background Adenosine receptor A2b (A2B adenosine receptor, A2BAR, ADORA2B) belongs to the adenosine receptor group of G protein-coupled receptors (GPCRs) (1-6). The adenosine receptors are divided into A1, A2, and A3 subtypes based on their ability to inhibit or stimulate adenyl cyclase (1-3). Within the A2 subtype, the high-affinity receptor is designated the A2A adenosine receptor, and the low-affinity receptor is the A2B adenosine receptor (1-3). Adenosine receptor A2b has been identified as a regulator of inflammation and vascular adhesion, primarily via signals from hematopoietic cells to the vasculature (3). Adenosine receptor A2b couples to G proteins to activate adenyl cyclase, leading to increased intracellular cAMP levels, as well as activation of phospholipase C (PLC), leading to increased intracellular calcium (4-6). Multiple mediators associated with inflammation, such as LPS, TNF-α, and IFN-γ, have been demonstrated to increase A2BAR expression in certain cell types (4-6). Alternate Names AA2BR; adenosine A2b receptor; Adenosine receptor A2b; ADORA2; ADORA2B Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 30-50 Source/Isotype: Rabbit IgG