CST, 2798S, ERp72 Antibody

Polyclonal Antibody for studying ERp72. Validated for Western Blotting. Highly specific and rigorously validated in-house, ERp72 Antibody (CST #2798) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ERp72 Antibody detects endogenous levels of total ERp72 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ERp72. Antibodies are purified by peptide affinity chromatography. Background Secretory proteins translocate into the endoplasmic reticulum (ER) during synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess thioredoxin homology domains, including ER stress protein 72 (ERp72), constitute the PDI family (3,4). ERp72 contains three thioredoxin homology domains (3) and plays a role in the formation and isomerization of disulfide bonds (3,4). Alternate Names Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; ER protein 70; ER protein 72; ERp-72; ERP70; ERP72; PDIA4; protein disulfide isomerase family A member 4; protein disulfide isomerase family A, member 4; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related); protein disulfide isomerase-associated 4; Protein disulfide-isomerase A4; testicular tissue protein Li 137 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 72 SOURCE: Rabbit