CST, 2803S, Survivin Antibody

Polyclonal Antibody for studying Survivin. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Survivin Antibody (CST #2803) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Survivin Antibody detects endogenous levels of total human survivin. This antibody does not cross-react with other types of apoptotic inhibitor proteins at physiological conditions. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human survivin. Antibodies are purified by protein A and peptide affinity chromatography. Background Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). Alternate Names API4; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat containing 5; baculoviral IAP repeat-containing 5; Baculoviral IAP repeat-containing protein 5; BIRC5; EPR-1; IAP4; Survivin; survivin variant 3 alpha Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 16 SOURCE: Rabbit