CST, 2804S, Hexokinase I Antibody

Polyclonal Antibody for studying Hexokinase 1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Hexokinase I Antibody (CST #2804) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Hexokinase I Antibody detects endogenous levels of total hexokinase I protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser340 of human hexokinase I protein. Antibodies are purified by peptide affinity chromatography. Background Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). Alternate Names brain form hexokinase; glycolytic enzyme; hexokinase; hexokinase 1; Hexokinase 1 isoform HKI variant; hexokinase IR; hexokinase type I; Hexokinase-1; Hexokinase-A; HK; HK I; HK1; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; NEDVIBA; RP79 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 102 SOURCE: Rabbit