CST, 2806S, WAVE-3 Antibody

Polyclonal Antibody for studying WAVE3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, WAVE-3 Antibody (CST #2806) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity WAVE-3 Antibody detects endogenous levels of total WAVE-3 protein. This antibody does not cross-react with WAVE-1 or WAVE-2. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human WAVE-3. Antibodies are purified using protein A and peptide affinity chromatography. Background Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8). Alternate Names Actin-binding protein WASF3; Brush-1; KIAA0900; Protein WAVE-3; SCAR3; Verprolin homology domain-containing protein 3; WAS protein family member 3; WAS protein family, member 3; WASF3; WASP family member 3; WASP family protein member 3; WASP family Verprolin-homologous protein 3; WAVE3; Wiskott-Aldrich syndrome protein family member 3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 65-70 SOURCE: Rabbit