CST, 28070T, Complexin-1/2 (D8A6E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying complexin-2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Complexin-1/2 (D8A6E) Rabbit Monoclonal Antibody (CST #28070) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Complexin-1/2 (D8A6E) Rabbit Monoclonal Antibody recognizes endogenous levels of total complexin-1 and complexin-2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro125 of human complexin-2 protein. Background Complexins are small soluble proteins composed of a central α-helical-structured domain surrounded by amino- and carboxy-terminal unstructured domains (1). These cytosolic proteins bind to t-SNAREs with low affinity and to assembled SNARE complexes with high affinity (1,2). Two isoforms, complexin-1 and complexin-2, are expressed in neuronal cells (3) where they regulate evoked and spontaneous exocytosis (4,5). Altered complexin expression resulting from RNAi-mediated knockdown (6) or gene invalidation (7) leads to alteration in spontaneous fusion events and neurotransmitter release, which reflects functions at both inhibitory and stimulatory synapses. Alternate Names 921-L; complexin 2; Complexin II; Complexin-2; CPLX2; CPX II; CPX-2; CPX2; Hfb1; MGC138492; synaphin 1; Synaphin-1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 14-16 Source/Isotype: Rabbit IgG