CST, 28109T, CD36 (E8B7S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD36 mouse. Validated for WB,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CD36 (E8B7S) Rabbit Monoclonal Antibody (CST #28109) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 86825 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD36 (E8B7S) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD36 protein and reacts with an epitope surrounding Ile410. Non-specific nucleolar background may be observed by immunofluorescence in methanol fixed samples. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with mouse CD36 recombinant protein. Background CD36 is a class B scavenger receptor composed of short amino-terminal and carboxy-terminal cytoplasmic domains, two transmembrane domains, and a large glycosylated extracellular domain (1-4). The CD36 receptor has many diverse ligands and cellular functions and is expressed by multiple cell types, including monocytes, macrophages, platelets, endothelial cells, adipocytes, and some epithelial cells (1). Binding of thrombospondin-1 (TSP-1) to CD36 facilitates the inhibition of angiogenesis by TSP-1 (5). CD36 also binds lipids and enables their transport into cells (6). In macrophages, CD36 acts as a receptor for oxidized LDL (Ox-LDL) and is responsible for Ox-LDL internalization, which contributes to development of atherosclerosis (7). The CD36 receptor participates in the innate immune response by acting as a pattern recognition receptor for lipid components of bacterial cell walls and fungal beta-glucans (8,9). CD36 likely influences signaling by interacting with other cell surface receptors including TLRs, integrins, and tetraspanins (8,10,11). Phorbol 12-myristate 13-acetate (PMA)/ 12-O-tetradecanoylphorbol-13-acetate (TPA) induces CD36 expression in the THP-1 monocyte cell line (12). Alternate Names Cd36; CD36 antigen; CD36 molecule; FAT; fatty acid translocase; glycoprotein IIIb; GPIIIB; GPIV; PAS IV; PAS-4; Platelet glycoprotein 4; platelet glycoprotein IV; Q08857; Sca; Scarb3 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 70-110 Source/Isotype: Rabbit IgG