CST, 28162T, Neutrophil Elastase (F4H3X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying neutrophil elastase. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Neutrophil Elastase (F4H3X) Rabbit Monoclonal Antibody (CST #28162) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 61891 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Neutrophil Elastase (F4H3X) Rabbit Monoclonal Antibody recognizes endogenous levels of total neutrophil elastase protein. This antibody cross-reacts with pro- and mature forms of neutrophil elastase protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with full-length recombinant protein specific to human neutrophil elastase protein. Background Neutrophil elastase is a hematopoietic serine protease that belongs to the chymotrypsin superfamily and plays a critical role in the innate immune function of mature neutrophils and monocytes (1,2). Neutrophil elastase is actively synthesized as an inactive zymogen in myelocytic precursor cells of the bone marrow, which then undergoes activation by limited proteolysis and sorting to primary (azurophil) storage granules of mature neutrophil granulocytes for regulated release (3,4). Research studies have shown that neutrophils play a significant role in mediating the inflammatory response through the release of neutrophil elastase, which activates pro-inflammatory cytokines and degrades components of the extracellular matrix and Gram-negative bacteria (5). Mutations in the gene encoding neutrophil elastase, , have been implicated in hematological diseases such as cyclic and severe congenital neutropenia, which is characterized by defects in promyelocyte maturation (6,7). Alternate Names Bone marrow serine protease; ELA2; ELANE; elastase 2, neutrophil; Elastase-2; elastase, neutrophil expressed; ELNE; GE; granulocyte-derived elastase; HLE; HNE; Human leukocyte elastase; leukocyte elastase; Medullasin; NE; Neutrophil elastase; PMN elastase; PMN-E; polymorphonuclear elastase; SCN1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG