CST, 2816S, Grp75 Antibody

Polyclonal Antibody for studying GRP75. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Grp75 Antibody (CST #2816) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Grp75 Antibody detects endogenous levels of total Grp75 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human Grp75. Antibodies are purified by peptide affinity chromatography. Background Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6). Alternate Names 75 kDa glucose regulated protein; 75 kDa glucose-regulated protein; catecholamine-regulated protein 40; CRP40; CSA; epididymis secretory sperm binding protein Li 124m; EVPLS; GRP-75; GRP75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9; heat shock 70kDa protein 9 (mortalin); heat shock protein family A (Hsp70) member 9; HEL-S-124m; HSPA9; HSPA9B; MGC4500; Mortalin; mortalin-2; mortalin, perinuclear; mortalin2; MOT; mot-2; MOT2; mt-HSP70; MTHSP75; p66-mortalin; PBP74; Peptide-binding protein 74; SAAN; SIDBA4; Stress-70 protein, mitochondrial Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 75 SOURCE: Rabbit