CST, 2820S, Ring1A Antibody

Polyclonal Antibody for studying Ring1A. Validated for Western Blotting. Highly specific and rigorously validated in-house, Ring1A Antibody (CST #2820) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ring1A Antibody detects endogenous levels of total Ring1A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Ring1A. Antibodies are purified by protein A and peptide affinity chromatography. Background Ring1A plays a role in polycomb group (PcG) protein function. PcG proteins are critically involved in transcriptional repression of Hox genes during development (1,2). PcG proteins form two distinct complexes: EED-EZH2 and the PRC complex, which is composed of at least Bmi1 and Ring1A/Ring1B. The EZH2-containing complex is responsible for the methylation of H3K27, and the PRC complex ubiquitylates H2A. EZH2 methylation is required prior to PRC ubiquitylation, and both are essential for Hox gene repression (1). It has recently been shown that PcG proteins silence a group of developmentally important regulator genes, referred to as bivalent genes (3). This regulation may be responsible for the ability of stem cells to self renew or switch to differentiate into multipotent progenitors. Aberrant epigenetic silencing by PcG proteins is also thought to be important in tumorigenesis (4). Alternate Names E3 ubiquitin-protein ligase RING1; Polycomb complex protein RING1; Really interesting new gene 1 protein; RING finger protein 1; RING-type E3 ubiquitin transferase RING1; RING1; RING1A; RNF1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 54 SOURCE: Rabbit