CST, 28284T, C9orf7/CACFD1 (E6E3J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Flower. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, C9orf7/CACFD1 (E6E3J) Rabbit Monoclonal Antibody (CST #28284) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity C9orf7/CACFD1 (E6E3J) Rabbit Monoclonal Antibody recognizes endogenous levels of total human C9orf7/CACFD1 protein. Based on the antigen sequence, this antibody is predicted to detect isoforms 1 and 2 of C9orf7/CACFD1 protein, but not isoforms 3 or 4. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu168 of human C9orf7/CACFD1 protein. Background C9orf7/Calcium Channel Flower Domain Containing 1 (CACFD1), also known as Flower (FWE), is the human homolog of the gene. In , expression of specific FWE membrane protein isoforms indicates the fitness of a cell during clonal selection and cell competition processes, which promotes the elimination of weaker cells from a growing population (1). In humans, there are four isoforms of C9orf7/CACFD1. Investigators have shown expression of human C9orf7/CACFD1 isoforms 1 and 3 indicates reduced cellular fitness compared to cells expressing isoforms 2 and 4, which provide a competitive advantage in survival. Moreover, human cancer cells show increased expression of isoforms 2 and 4 that confers a competitive growth advantage for cancer cells. Inhibition of C9orf7/CACFD1 expression reduces tumor growth and metastasis, and induces sensitivity to chemotherapy (2-5). Alternate Names C9orf7; CACFD1; calcium channel flower domain containing 1; Calcium channel flower domain-containing protein 1; Calcium channel flower homolog; D9S2135; FLOWER; FLOWR Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 19 Source/Isotype: Rabbit IgG