CST, 28365S, Hexokinase I (C35C4) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3095)

Monoclonal Antibody for studying Hexokinase 1. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Hexokinase I (C35C4) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3095) (CST #28365) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Hexokinase I (C35C4) Rabbit mAb (InTraSeq™ 3' Conjugate 3095) detects endogenous levels of total hexokinase I protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu108 of human hexokinase I protein. Background Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). Alternate Names brain form hexokinase; glycolytic enzyme; hexokinase; hexokinase 1; Hexokinase 1 isoform HKI variant; hexokinase IR; hexokinase type I; Hexokinase-1; Hexokinase-A; HK; HK I; HK1; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; NEDVIBA; RP79 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG