CST, 2836S, Neurofilament-H (RMdO 20) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Neurofilament-H. Validated for Western Blotting,Simple Western™,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Neurofilament-H (RMdO 20) Mouse Monoclonal Antibody (CST #2836) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Frozen): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 92028 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity Neurofilament-H (RMdO 20) Mouse Monoclonal Antibody detects endogenous levels of total Neurofilament-H protein. Species cross-reactivity for IF-IC is rodent only. This antibody has been reported to detect NFM and NFH in human samples but only NFH in mouse, rat or bovine samples (Lee, V.M. et al., 1988). Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with rat neurofilament. Background The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). Studies of NFH (-/-) mice suggest that NFH modulates ion channel functions in large myelinated fibers (5). Alternate Names 200 kDa neurofilament protein; CMT2CC; KIAA0845; NEFH; Neurofilament H; neurofilament heavy; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; NF-H; NFH Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 180-220 Source/Isotype: Mouse IgG1