CST, 2838T, Neurofilament-M (RMO 14.9) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Neurofilament-M. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Neurofilament-M (RMO 14.9) Mouse Monoclonal Antibody (CST #2838) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Neurofilament-M (RMO 14.9) Mouse Monoclonal Antibody detects endogenous levels of total Neurofilament-M protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with rat neurofilament, medium chain. Background The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). Alternate Names 160 kDa neurofilament protein; NEF3; NEFM; Neurofilament 3; Neurofilament M; neurofilament medium; Neurofilament medium polypeptide; Neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; NF-M; NFM Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Mouse IgG1