CST, 28405T, LRSAM1 (D1O5S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LRSAM1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, LRSAM1 (D1O5S) Rabbit Monoclonal Antibody (CST #28405) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LRSAM1 (D1O5S) Rabbit Monoclonal Antibody recognizes endogenous levels of total LRSAM1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro143 of human LRSAM1 protein. Background Leucine-rich repeat and sterile alpha motif-containing protein 1 (LRSAM1, hTAL1) is a multi-domain-containing E3 ubiquitin-ligase involved in the regulation of cell adhesion. The LSRAM1 protein contains amino-terminal leucine-rich repeats (LRR), an ezrin-radixin-moesin (ERM) domain, a coiled-coil region, a sterile alpha motif (SAM) domain, and a carboxy-terminal RING finger domain. Research studies demonstrate that LRSAM1 participates in the endosomal sorting of proteins by regulating the ubiquitination of Tsg101, a component of the ESCRT-I endosomal sorting complex (1). LSRAM1 ubiquitin ligase activity plays a critical role in promoting the ubiquitin-dependent autophagic clearance of pathogenic bacteria (2). Mutations in the corresponding gene can contribute to a form of Charcot-Marie-Tooth (CMT2P) disease that is characterized by peripheral nervous system axonal neuropathy (3,4). Alternate Names CMT2P; E3 ubiquitin-protein ligase LRSAM1; FLJ31641; hTAL; leucine rich repeat and sterile alpha motif containing 1; Leucine-rich repeat and sterile alpha motif-containing protein 1; LRSAM1; LRSM1; RIFLE; RING finger leucine repeat rich; RING-type E3 ubiquitin transferase LRSAM1; TAL; Tsg101-associated ligase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 84 Source/Isotype: Rabbit IgG