CST, 28409S, TAF15 (D8V6Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TAF15. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, TAF15 (D8V6Q) Rabbit Monoclonal Antibody (CST #28409) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:100 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TAF15 (D8V6Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total TAF15 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg584 of human TAF15 protein. Background TATA-binding protein (TBP)-associated factor 15 (TAF15) is a member of the multifunctional FUS, EWS, and TAF15 (FET) family of proteins that bind both DNA and RNA and may be important for transcriptional regulation and RNA processing (1,2). Research studies have shown that FET family proteins, including TAF15, can be found as oncogenic fusion proteins with transcription factors in various cancers. The fusion protein consists of the FET protein amino-terminal transcription activation domain and the transcription factor DNA binding domain, creating an aberrant transcription factor with potent transactivation potential (1-3). TAF15 can be part of a subset of the transcription factor IID (TFIID) complex, indicating that it may form a transcription pre-initiation complex at active genes, even if it is not considered a canonical TAF (4,5). Additional studies suggest roles for TAF15 in RNA splicing through its association with U1 small nuclear RNA and in regulation of cell cycle regulatory gene expression through a mechanism involving miRNA biosynthesis (6,7). TAF15 aggregation and amyloid filament formation have been reported within the brain of individuals with frontotemporal lobar degeneration-FUS (FTLD-FUS) (8). Alternate Names 68 kDa TATA-binding protein-associated factor; hTAFII68; Npl3; RBP56; RBP56/CSMF fusion; RNA-binding protein 56; TAF(II)68; TAF15; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa; TAF2N; TAFII68; TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56); TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA-binding protein-associated factor 2N; TATA-box binding protein associated factor 15; TBP-associated factor 15 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 77 Source/Isotype: Rabbit IgG