CST, 2841T, Phospho-C/EBP alpha (Ser21) Antibody

Polyclonal Antibody for studying C/EBP-alpha (Ser21) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-C/EBP alpha (Ser21) Antibody (CST #2841) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-C/EBP alpha (Ser21) Antibody detects endogenous levels of C/EBPα only when phosphorylated at serine 21. This antibody does not cross-react with other phosphorylated C/EBP isoforms. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human C/EBPα. Antibodies are purified by protein A and peptide affinity chromatography. Background CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε, and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-I stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin-induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 seems to be required for adipogenesis (4). Alternate Names C/EBP alpha; C/EBP-alpha; CCAAT enhancer binding protein alpha; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer-binding protein alpha; CEBP; CEBPA Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 45 SOURCE: Rabbit