CST, 28470S, CD127/IL-7R alpha (A7R34) Rat Monoclonal Antibody (APC Conjugate)

Monoclonal Antibody for studying IL7R mouse. Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD127/IL-7R alpha (A7R34) Rat Monoclonal Antibody (APC Conjugate) (CST #28470) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.25 μg of antibody per test. Flow Cytometry (Live): 1:80 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity CD127/IL-7R alpha (A7R34) Rat Monoclonal Antibody (APC Conjugate) recognizes endogenous levels of total CD127/IL-7Rα protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background The IL-7 receptor (IL-7R) is comprised of two protein subunits, CD127/IL-7Rα (IL-7Ralpha) and the common gamma chain (CD132), which is the major signaling component for several cytokines including IL-2, IL-4, IL-9, IL-15, and IL-21 (1). CD127/IL-7Rα is a transmembrane protein belonging to the cytokine receptor homology class 1 (CRH1) and is expressed by a wide variety of cells including immature B cells, thymic natural killer cells, bone marrow stromal cells, and T cells (5-6). On its own, CD127/IL-7Rα functions as a receptor for two cytokine receptor complex signaling cascades: IL-7 and thymic stromal lymphopoietin (TSLP) (2). IL-7 signaling contributes to T cell development and homeostasis whereas TSLP receptor signaling contributes to dendritic cell activation and B cell development. IL-7 signaling is an essential component in regulating the homeostasis of naive and memory T cells as differential expression of CD127/IL-7Rα is observed on naive and activated T cells, which occurs following TCR activation. Specifically, CD127/IL-7Rα expression is downregulated on activated T cells and the subsequent re-expression of CD127/IL-7Rα on these cells is indicative of cells that will differentiate into memory T cells (3-4). The A7R34 antibody is widely used to identify CD127/IL-7Rα expression on both B and T cells (6). Alternate Names CD127; IL-; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; IL-7Ralpha; Il7r; IL7RA; interleukin 7 receptor; interleukin 7 receptor alpha chain; Interleukin-7 receptor subunit alpha; MGC107557 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2a kappa