CST, 2848S, Vav2 (C64H2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VAV2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Vav2 (C64H2) Rabbit Monoclonal Antibody (CST #2848) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Vav2 (C64H2) Rabbit Monoclonal Antibody detects endogenous levels of total Vav2 protein. The antibody is not expected to cross-react with Vav1 or Vav3 based on the sequence of the immunogenic peptide. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Vav2. Background Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3). Alternate Names Guanine nucleotide exchange factor VAV2; oncogene VAV2; Protein vav-2; vav 2 guanine nucleotide exchange factor; vav 2 oncogene; vav guanine nucleotide exchange factor 2; VAV-2; VAV2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG