CST, 28492S, IRF-9 (D8G7H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IRF-9. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, IRF-9 (D8G7H) Rabbit Monoclonal Antibody (CST #28492) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity IRF-9 (D8G7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRF-9 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro362 of human IRF-9 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). In response to type I interferons (e.g., IFN-α, IFN-β), interferon regulatory factor 9 (IRF-9, ISGF3γ) assembles into a protein complex with Stat1 and Stat2 and translocates to the nucleus where it induces transcription of interferon response genes by binding to ISREs (3,4). Expression of IRF-9 is also induced by type I interferons (5). Alternate Names IFN-alpha-responsive transcription factor subunit; Interferon regulatory factor 9; Interferon-stimulated gene factor 3 gamma; interferon-stimulated transcription factor 3, gamma (48kD); interferon-stimulated transcription factor 3, gamma 48kDa; IRF-9; IRF9; ISGF-3 gamma; ISGF3; ISGF3 p48 subunit; ISGF3G; p48; Transcriptional regulator ISGF3 subunit gamma Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG