CST, 2853S, Phospho-ATR (Ser428) Antibody

Polyclonal Antibody for studying ATR (Ser428) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-ATR (Ser428) Antibody (CST #2853) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-ATR (Ser428) Antibody detects endogenous levels of ATR only when phosphorylated at serine 428. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser428 of human ATR. Antibodies are purified by protein A and peptide affinity chromatography. Background Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. ATR was long thought to exist in a constitutively active state in cells, with DNA damage-induced signaling occurring via recruitment of ATR to single stranded DNA and sites of replication stress. Phosphorylation of ATR at serine 428 in response to UV-induced DNA damage has been suggested as a means of activating ATR (4,5). Recent work has shown autophosphorylation of ATR at threonine 1989. Like ATM Ser1981, phosphorylation of ATR Thr1989 occurs in response to DNA damage, indicating that phosphorylation at this site is important in ATR-mediated signaling (6,7). Alternate Names ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR; ATR serine/threonine kinase; FCTCS; FRAP-related protein 1; FRAP-related protein-1; FRP1; MEC1; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Rad3 related protein; SCKL; SCKL1; Serine/threonine-protein kinase ATR Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 300 SOURCE: Rabbit