CST, 28556S, PAX8 (BC12) Mouse Monoclonal Antibody

Monoclonal Antibody for studying PAX8. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PAX8 (BC12) Mouse Monoclonal Antibody (CST #28556) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in a Tris-based buffer with ~1% BSA and less than 0.1% sodium azide. Stable for 12 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity PAX8 (BC12) Mouse Monoclonal Antibody recognizes endogenous levels of PAX8 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with the full-length human recombinant PAX8 protein. Background Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2). PAX8 is involved in the development of thyroid follicular cells and the expression of thyroid-specific genes (5). Investigators have associated mutations in the gene with thyroid dysgenesis, thyroid follicular carcinomas, and atypical follicular thyroid adenomas (6,7). Alternate Names paired box 8; Paired box protein Pax-8; paired domain gene 8; PAX8 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1