CST, 2857T, Phospho-DUSP1/MKP1 (Ser359) (125E2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DUSP1 (Ser359) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-DUSP1/MKP1 (Ser359) (125E2) Rabbit Monoclonal Antibody (CST #2857) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-DUSP1/MKP1 (Ser359) (125E2) Rabbit Monoclonal Antibody detects endogenous levels of DUSP1 only when phosphorylated at serine 359. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser359 of human DUSP1. Background MAP kinases are inactivated by a family of dual-specificity protein phosphatases (DUSP) that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli and cellular localization. DUSP1/MKP1 (MAP Kinase phosphatase 1) is primarily localized in the nucleus, and has broad substrate specificity towards p44/42 MAPK, p38 MAPK and SAPK/JNK (1-3). DUSP1's transcription and activity are tightly regulated. First, DUSP1 is transcriptionally induced through p44/42 MAPK, p53 and Jak2 (2,4,5). Second, DUSP1 is phosphorylated by p44/42 MAPK at Ser359 and Ser364 in its carboxy-terminal region, which inhibits DUSP1 degradation through the ubiquitin pathway (6). Alternate Names CL 100; CL100; dual specificity phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1; DUSP1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein-tyrosine phosphatase CL100; PTPN10; serine/threonine specific protein phosphatase; VH1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG