CST, 28610T, NGF (E2S9D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NGF mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NGF (E2S9D) Rabbit Monoclonal Antibody (CST #28610) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NGF (E2S9D) Rabbit Monoclonal Antibody recognizes human and mouse recombinant NGF, and exogenously expressed NGF protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse NGF protein. Background Nerve growth factor (NGF) is a small, secreted protein and member of the neurotrophin family of growth factors that promote neuronal cell survival and differentiation (1). Producing cells release NGF that bind and activate TrkA high affinity receptors to mediate NGF-driven signaling. NGF also binds to low affinity p75 (NTR) receptors, which belong to the death receptor family (2). Although NGF has been classically described as favoring neuron survival and differentiation, NGF can promote apoptosis in cells that contain p75 (NTR) and lack TrkA. NGF can induce neuron death in a variety of neurodegenerative conditions, including Alzheimer's disease (3). Besides its neurotrophic actions, NGF has an effect on non-neuronal cells and may help mediate inflammation, angiogenesis, and stimulate breast cancer cell growth (4-6). NGF signaling is looking increasingly promising as potential drug targets for diseases. Alternate Names Beta-nerve growth factor; Beta-NGF; nerve growth factor; nerve growth factor, beta; Ngf; Ngfb Specification REACTIVITY: H M SENSITIVITY: Recombinant and Transfected MW (kDa): Precursor 28 - Mature 13 Source/Isotype: Rabbit IgG