CST, 28630S, Phospho-Syntaxin 17 (Ser202) Antibody

Polyclonal Antibody for studying STX17 (Ser202) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Syntaxin 17 (Ser202) Antibody (CST #28630) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Syntaxin 17 (Ser202) Antibody recognizes transfected levels of syntaxin 17 protein only when phosphorylated at Ser202. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser202 of human syntaxin 17 protein. Antibodies were purified by peptide affinity chromatography. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes, including development, differentiation, neurodegeneration, infection, and cancer (3). Syntaxin 17/STX17 is a SNARE factor recruited to autophagosomes and required for autophagosome fusion to lysosomes. Syntaxin 17 interacts with SNAP29 (Qbc-SNARE synaptosome-associated protein 29) and the lysosomal factor VAMP8 (R-SNARE vesicle-associated membrane protein 8), as well as BRUCE, an inhibitor of apoptosis (IAP) protein, which is also involved in autophagosome/lysosome fusion (4,5).Syntaxin 17 promotes initiation of PINK1/Parkin-independent mitophagy, which is regulated by depletion of the mitochondrial outer membrane protein Fis1 (6). Phosphorylation of Syntaxin 17 at Ser202 by TBK1 promotes the formation of the mammalian pre-autophagosomal structure (mPAS) and interaction with core components Atg13 and FIP200. Alternate Names FLJ20651; MGC102796; MGC126613; MGC126615; STX17; syntaxin 17; Syntaxin-17 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 40-42 SOURCE: Rabbit