CST, 28692T, 5-Methylcytosine (5-mC) (D3S2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Immunofluorescence (Immunocytochemistry),DNA Dot Blot. Available in 2 sizes. Highly specific and rigorously validated in-house, 5-Methylcytosine (5-mC) (D3S2Z) Rabbit Monoclonal Antibody (CST #28692) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:1600 DNA Dot Blot: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), DNA Dot Blot Specificity / Sensitivity 5-Methylcytosine (5-mC) (D3S2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of 5-methylcytosine. This antibody has been validated using ELISA, dot blot, and MeDIP assays and shows high specificity for 5-methylcytosine. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with 5-methylcytidine. Background Methylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development (1,2). 5-methylcytosine is a repressive epigenetic mark established by two enzymes, DNMT3a and DNMT3b, and is maintained by DNMT1 (3, 4). 5-methylcytosine was originally thought to be passively depleted during DNA replication. However, subsequent studies have shown that Ten-Eleven Translocation (TET) proteins TET1, TET2, and TET3 can catalyze the oxidation of methylated cytosine to 5-hydroxymethylcytosine (5-hmC) (5). Additionally, TET proteins can further oxidize 5-hmC to form 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC), both of which are excised by thymine-DNA glycosylase (TDG), effectively linking cytosine oxidation to the base excision repair pathway and supporting active cytosine demethylation (6,7). Normally DNA methylation occurs in a bimodal fashion, such that CpG dinucleotides are largely methylated across the genome, except in short stretches of CpG-rich sequences associated with gene promoters, known as CpG-islands, where methylation is virtually absent (8). Cancer cell genomes often undergo global hypomethylation, while CpG-islands become hypermethylated, causing their associated promoters to become repressed (9). There is evidence that a number of aberrantly hypermethylated CpG-islands found in carcinomas occur at tumor suppressor genes such as RB1, MLH1, and BRCA1 (10). Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG