CST, 2869T, Syntaxin 6 (C34B2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STX6. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Syntaxin 6 (C34B2) Rabbit Monoclonal Antibody (CST #2869) is ready to ship. Product Usage Information Western Blotting: 1:2000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Syntaxin 6 (C34B2) Rabbit Monoclonal Antibody detects endogenous levels of total syntaxin 6 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr140 of mouse syntaxin 6 protein. Background Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (1,2). The protein has an amino-terminal H1 domain followed by an H2 SNARE domain and a carboxy-terminal membrane anchor (3). Syntaxin 6 protein is localized to the trans-Golgi and within endosomes and regulates membrane trafficking by partnering with a variety of other SNARE proteins (3-5). Depending on cell type and SNARE parter, syntaxin 6 is involved in the regulation of GLUT4 trafficking, neutrophil exocytosis and granule secretion (6-10). Alternate Names STX6; syntaxin 6; Syntaxin-6; Syntaxin6 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG