CST, 28712S, FoxK2 (E7C5H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FOXK2. Validated for WB,WB,IP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, FoxK2 (E7C5H) Rabbit Monoclonal Antibody (CST #28712) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity FoxK2 (E7C5H) Rabbit Monoclonal Antibody recognizes endogenous levels of total FoxK2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala414 of human FoxK2 protein. Background Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA-binding domain called a forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech/language disorders, and cancer (3,4). FoxK1 and FoxK2 belong to one of the subfamilies of Fox proteins and contain a forkhead-associated (FHA) domain and a conserved forkhead DNA-binding domain (5). FoxK2 was initially identified as a regulator of IL-2 transcription (6). Studies have shown that FoxK2 binds to DNA harboring G/T mismatches and might be involved in DNA mismatch repair (7). FoxK2 also mediates the oncogenic function of adenovirus (Adv) oncoprotein E1A and papillomavirus E6 proteins via association with these proteins (8). Moreover, FoxK2 promotes AP-1-mediated transcriptional regulation by enhancing the recruitment of AP-1 to chromatin (9). FoxK2 is phosphorylated at Ser368 and Ser423 in a CDK1-cyclin B-dependent manner and is involved in cell cycle regulation (10). Alternate Names Cellular transcription factor ILF-1; forkhead box K2; Forkhead box protein K2; FOXK1; FOXK2; G/T-mismatch specific binding protein; ILF; ILF-1; ILF1; interleukin enhancer binding factor 1; Interleukin enhancer-binding factor 1; nGTBP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 79 Source/Isotype: Rabbit IgG