CST, 28716T, SRCAP (E9V8O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SRCAP. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, SRCAP (E9V8O) Rabbit Monoclonal Antibody (CST #28716) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity SRCAP (E9V8O) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRCAP protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro911 of human SRCAP protein. Background Snf2-Related CBP Activator Protein (SRCAP) is an ATPase that exists in a large complex that activates transcription by enhancing CBP activity and by depositing H2A.Z into nucleosomes (1-3). SRCAP can enhance nuclear receptor function along with CARM-1 and GRIP-1 (4). In prostate cancer, SRCAP associates with androgen receptor to control prostate specific antigen expression and increase cell growth (5). During double-stranded break repair, SRCAP is required for DNA-end resection and RAD51 recruitment (6). Mutations in SRCAP result in a developmental disorder called Floating-Harbor syndrome (7,8). SRCAP also plays a role in viral infection as it has been shown to interact with various viral proteins (9,10). Alternate Names domino homolog 1; Domino homolog 2; DOMO1; EAF1; FLHS; FLJ44499; Helicase SRCAP; KIAA0309; Snf2 related CREBBP activator protein; Snf2-related CBP activator; Snf2-related CBP activator protein; Snf2-related CREBBP activator protein; SRCAP; Swi2/Snf2-related ATPase homolog, domino homolog 1; SWR1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 400 Source/Isotype: Rabbit IgG