CST, 28755S, Thymidine Kinase 1 (E2H7Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Thymidine kinase. Validated for WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Thymidine Kinase 1 (E2H7Z) Rabbit Monoclonal Antibody (CST #28755) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 34017 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Thymidine Kinase 1 (E2H7Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total thymidine kinase 1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro209 of human thymidine kinase 1 protein. Background Thymidine kinases play a critical role in generating the DNA synthetic precursor deoxythymidine triphosphate (dTTP) by catalyzing the phosphotransfer of phosphate from ATP to deoxythymidine (dT) and thymidine (T) in the cell. There are two known thymidine kinases, cytoplasmic thymidine kinase 1 (TK1) and mitochondrial thymidine kinase 2 (TK2) (1,2). Unlike TK2, which is not modulated by the cell cycle, TK1 expression and activity is regulated in a cell cycle-dependent manner, accumulating during G1-phase to peak levels in S-phase before being degraded prior to cell division (3,4). Stability, but not activity, may be regulated via phosphorylation of TK1 at Ser13 by Cdc2 and/or Cdk2, but the precise mode of regulation remains elusive (5). These observations indicate that TK1 might be a useful marker of cell proliferation; however, recent studies have shown that TK1 plays a more significant role in the DNA damage response (6). Genotoxic stress promotes increased TK1 expression and kinase activity resulting in reduced cellular apoptosis and enhanced DNA repair efficiency (6). More importantly, numerous studies show that TK1 expression and activity are upregulated during neoplasia and disease progression in humans, and increased serum levels of TK1 correlate with poor prognosis and decreased survival in patients with various types of advanced tumors (7-12). Alternate Names cytosolic thymidine kinase; epididymis secretory sperm binding protein; KITH; thymidine kinase 1; thymidine kinase 1 soluble isoform; thymidine kinase 1, soluble; thymidine kinase-1; Thymidine kinase, cytosolic; TK; TK1; TK2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG