CST, 28764S, dsRNA (K1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, dsRNA (K1) Mouse Monoclonal Antibody (CST #28764) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Lyophilized from a PBS solution that does not contain any stabilizers or preservatives. The lyophilized antibody is stable for 24 months when stored at -20°C. Reconstitute using 200 μl of sterile distilled water to create a 1 mg/ml solution. A slight precipitate may be present, but will not interfere with antibody performance. Recommended to centrifuge the reconstituted antibody and use the supernatant. Once reconstituted, this product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles . Protocol Available protocols: Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity dsRNA (K1) Mouse Monoclonal Antibody recognizes dsRNA where the length of the helix is greater than or equal to 40 base pairs (bp). The ability to recognize dsRNA is independent of the sequence and nucleotide composition of the antigen. The K1 clone shows a higher affinity to poly(I:C) than to other dsRNA antigens (6). Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a nucleic acid sequence. Background Double-stranded RNA (dsRNA) is produced during the replication cycle of a broad range of viruses and can trigger the innate immune response. dsRNA is present if cells are infected with a dsRNA virus or it can be generated during the course of single-stranded RNA (ssRNA) virus replication. Host cells do not produce dsRNA so the innate immune response can differentiate between host and viral RNAs by the presence of dsRNA (1). The innate immune response is controlled by several classes of germline-encoded pattern-recognition receptors (PRRs), including retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), Toll-like receptors (TLRs), and NOD-like receptors (NLRs) that sense viral components, such as dsRNA, ssRNA, and DNA (2,3). Activation of PRRs by dsRNA leads to production of type I interferons (IFNs) and proinflammatory cytokines, triggering the adaptive immune response (2,3). Understanding how antiviral responses are initiated, the counter strategies that viruses adopt, and exploring the viral life cycle gives researchers a better comprehension of how to treat infections of different viral diseases (4,5). Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Mouse IgG2a kappa