CST, 2877S, FE65 Antibody

Polyclonal Antibody for studying FE65. Validated for Western Blotting. Highly specific and rigorously validated in-house, FE65 Antibody (CST #2877) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FE65 Antibody detects endogenous levels of FE65. This antibody does not cross-react with FE65L1 or FE65L2. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human FE65. Antibodies are purified by protein A and peptide affinity chromatography. Background FE65, FE65L1, and FE65L2 are members of the FE65 protein family. FE65 is an adaptor protein with protein-protein interaction domains, including a WW domain followed by two phosphotyrosine interaction domains (PID1 and PID2) (1). Amyloid-β (Aβ) precursor protein (APP) binds to PID2 and undergoes sequential cleavage. First, α-/β secretases cleave and release the ectodomain into the extracellular environment. Subsequent processing by the γ-secretase complex results in the APP intracellular domain (AICD) and the Aβ peptides. The latter Aβ fragments form the main components of amyloid plaques in patients with Alzheimer's disease (2). FE65 family members can regulate APP processing, resulting in elevated levels of Aβ (3). Double knockout mice of FE65 and FE65L1 display a phenotype that occurs in animals lacking APP family members, supporting a functional interaction between FE65 and APP (4). Alternate Names adaptor protein FE65a2; amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65); Amyloid beta A4 precursor protein-binding family B member 1; amyloid beta A4 precursor protein-binding, family B, member 1; Amyloid beta precursor protein binding family B member 1; Amyloid-beta A4 precursor protein-binding family B member 1; APBB1; FE65; MGC:9072; Protein Fe65; RIR; stat-like protein Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit