CST, 2879S, LITAF Antibody

Polyclonal Antibody for studying LITAF. Validated for Western Blotting. Highly specific and rigorously validated in-house, LITAF Antibody (CST #2879) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LITAF Antibody dectects endogenous levels of total human LITAF protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LITAF protein. Antibodies were purified by peptide affinity chromatography. Background Lipopolysaccharide-induced TNF factor (LITAF), also known as PIG7 and SIMPLE, is a protein that contributes to the regulation of the inflammatory cytokine TNF-α (1-3). LITAF was identified as a transcription factor involved in LPS-induced TNF-α expression through interaction with the TNF-α promoter (1,4). The same protein, called PIG7, was independently described in a model for p53 regulation (2). A putative alternative spliced form of LITAF, named SIMPLE, encodes a protein with a unique carboxyl terminus (3). Studies on LITAF-deficient mice demonstrate that LITAF plays a significant role in the regulation of several inflammatory cytokines in response to LPS (5). LITAF regulation can occur through phosphorylation by p38α via the TLR pathway that leads to its nuclear translocation (5). Mutation in the gene has been associated with an autosomal dominant demyelinating form of Charcot-Marie-Tooth disease (6). Alternate Names FLJ38636; lipopolysaccharide induced TNF factor; lipopolysaccharide-induced TNF factor; lipopolysaccharide-induced TNF-alpha factor; Lipopolysaccharide-induced tumor necrosis factor-alpha factor; LITAF; LPS-induced TNF-alpha factor; MGC116698; MGC116700; MGC116701; MGC125274; MGC125275; MGC125276; p53-induced gene 7 protein; PIG7; SIMPLE; Small integral membrane protein of lysosome/late endosome; TP53I7; tumor protein p53 inducible protein 7 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 23 SOURCE: Rabbit