CST, 28825T, GATM (F6S8W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GATM. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, GATM (F6S8W) Rabbit Monoclonal Antibody (CST #28825) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity GATM (F6S8W) Rabbit Monoclonal Antibody recognizes endogenous levels of total GATM protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GATM protein. Background Mitochondrial glycine amidinotransferase (GATM), also known as arginine:glycine amidinotransferase (AGAT), catalyzes the conversion of glycine and L-arginine to guanidinoacetate and L-ornithine, the rate-limiting step of creatine biosynthesis (1-3). Mouse model studies of obesity-accelerated breast cancer show that this enzyme and creatine biosynthesis in adipocytes are essential to breast cancer progression driven by obesity (2). In addition, synthesis of creatine by GATM or dietary uptake of creatine promotes tumor metastasis in an orthotopic mouse model (3). Moreover, GATM expression increases in metastases of various cancers, such as colorectal cancer and melanoma (3). Furthermore, creatine plays a role in neurons and its function requires GATM and SLC6A8, a creatine transporter (4). Alternate Names AGAT; AT; CCDS3; FRTS1; GATM; glycine amidinotransferase; glycine amidinotransferase (L-arginine:glycine amidinotransferase); Glycine amidinotransferase, mitochondrial; L-arginine:glycine amidinotransferase; testicular secretory protein Li 19; Transamidinase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG