CST, 2886S, ERp44 Antibody

Polyclonal Antibody for studying ERp44. Validated for Western Blotting. Highly specific and rigorously validated in-house, ERp44 Antibody (CST #2886) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ERp44 Antibody detects endogenous levels of total ERp44 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ERp44. Antibodies are purified by protein A and peptide affinity chromatography. Background Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum resident protein 44 (ERp44), constitute the PDI family (2). ERp44 is induced upon ER stress and is linked to Ero1-Lα and Ero1-Lβ through mixed disulfide bonds (3). ERp44 was shown to mediate the ER localization of Ero1-Lα (4). Alternate Names endoplasmic reticulum protein 44; Endoplasmic reticulum resident protein 44; endoplasmic reticulum resident protein 44 kDa; epididymis secretory sperm binding protein; ER protein 44; ERP44; KIAA0573; PDIA10; protein disulfide isomerase family A, member 10; thioredoxin domain containing 4 (endoplasmic reticulum); thioredoxin domain-containing protein 4; TXNDC4 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 44 SOURCE: Rabbit