CST, 28897S, Phospho-IGF-I Receptor beta (Tyr1316) Antibody

Polyclonal Antibody for studying IGF1R (Tyr1346) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-IGF-I Receptor beta (Tyr1316) Antibody (CST #28897) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-IGF-I Receptor beta (Tyr1316) Antibody detects endogenous levels of IGF-I receptor only when phosphorylated at Tyr1316. This antibody may also cross-react with other overexpressed, related tyrosine-phosphorylated tyrosine kinases. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1316 of human IGF-I receptor. Antibodies are purified by protein A and peptide affinity chromatography. Background Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). Phosphorylation of IGF-I receptor on Tyr1346 (equivalent to Tyr1316 in mature protein) was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS/MS platform for phosphorylation site discovery (9). Phosphorylation of IGF-I receptor on Tyr1346 was also reported by several other labs in select carcinoma cell lines (10,11). Alternate Names CD221; IGF-I receptor; IGF1R; IGFIR; IGFR; insulin like growth factor 1 receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 95 SOURCE: Rabbit