CST, 2889S, Notch3 Antibody

Polyclonal Antibody for studying Notch3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Notch3 Antibody (CST #2889) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Notch3 Antibody detects endogenous levels of total Notch3 protein. This antibody recognizes both full-length (FL) Notch3 at 270 kDa and a truncated protein (NTM) containing a short extracellular region, the transmembrane domain and the intracellular region at 90 kDa. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2311 of human notch3. Antibodies were purified by protein A and peptide affinity chromatography. Background Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4). Notch3 is a member of notch family and processed similar to notch1 (5). It is expressed primarily in arterial smooth muscle cells (SMC). Mutations altering the number of cysteine residues in the notch3 extracellular region are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a hereditary angiopathy leading to strokes and dementia in adults (6-8). Recent studies indicates that notch3 is overexpressed in many types of cancers (9-11). Alternate Names CADASIL; CADASIL1; CASIL; IMF2; LMNS; Neurogenic locus notch homolog protein 3; NOTC3; Notch 3; Notch 3 extracellular truncation; Notch 3 intracellular domain; Notch homolog 3; Notch homolog 3 (Drosophila); notch receptor 3; NOTCH3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90, 270 SOURCE: Rabbit