CST, 28903SF, Bax (D3R2M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Bax mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Bax (D3R2M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #28903) is ready to ship. Product Usage Information This product is the carrier free version of product #14796. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14796 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Bax (D3R2M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Bax protein in rodent samples. This antibody can detect human Bax protein by western blot, but not by other applications. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu70 of mouse Bax protein. Background Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9, and initiation of the caspase activation pathway for apoptosis (3,4). Alternate Names Apoptosis regulator BAX; Bax; BCL2-associated X protein Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG