CST, 2891S, Calreticulin Antibody

Polyclonal Antibody for studying calreticulin. Validated for Western Blotting. Highly specific and rigorously validated in-house, Calreticulin Antibody (CST #2891) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Calreticulin Antibody detects endogenous levels of total calreticulin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Gln65 of human calreticulin. Antibodies are purified by peptide affinity chromatography. Background Calcium is a universal signaling molecule involved in many cellular functions, such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium-binding chaperones, such as calnexin and calreticulin, and is released via the IP receptor channel (1). Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation (2,3). Alternate Names autoantigen Ro; CALR; Calregulin; Calreticulin; calreticulin); cC1qR; CRP55; CRT; CRTC; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; HEL-S-99n; RO; Sicca syndrome antigen A (autoantigen Ro; SSA Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit