CST, 28938T, Tau 3R (F8P1G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tau iso2/Tau iso5/Tau iso4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Tau 3R (F8P1G) Rabbit Monoclonal Antibody (CST #28938) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24636 . Protocol Available protocols: Western Blotting Specificity / Sensitivity Tau 3R (F8P1G) Rabbit Monoclonal Antibody recognizes exogenously expressed tau isoforms 2, 4, and 5 (tau 3R). This antibody does not cross-react with tau isoforms 6, 7, and 8 protein (tau 4R). Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln218 of human tau isoform 2 protein. Background Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). Alternative splicing of exon 10 results in the expression of two groups of tau: three-repeat and four-repeat tau. Isoforms 2, 4, and 5 express three microtubule binding repeat domains (tau 3R) while isoforms 6, 7, and 8 express four microtubule binding repeat domains (tau 4R) (4). Expression of tau 3R and tau 4R in cells can be different in mild or pathological conditions. For example, tau 3R is preferentially expressed in Pick's disease (PiD) and corticobasal degeneration (CBD), while tau 3R and tau 4R are equally expressed in AD (5,6). The repeat-dependent tau has a different pattern of phosphorylation in different diseases and also has the ability and patterns of aggregation (7-9). Alternate Names DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPT; MAPTL; MGC138549; microtubule associated protein tau; Microtubule-associated protein tau; MSTD; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; TAU; Tau iso2; Tau iso4; Tau iso5 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 50-80 Source/Isotype: Rabbit IgG