CST, 28944SF, SV2A (D1L8S) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SV2A. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, SV2A (D1L8S) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #28944) is ready to ship. Product Usage Information This product is the carrier free version of product #66724. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 66724 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SV2A (D1L8S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SV2A protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe516 of human SV2A protein. Background SV2s are a family of synaptic vesicle proteins expressed in both neurons and endocrine cells. SV2s function in the regulation of synaptic vesicle traffic, cytoplasmic Ca levels in the nerve terminal during repetitive stimulation and the facilitation of synaptic transmission. There are three isoforms of SV2: SV2A, SV2B and SV2C. Each of these isoforms are structured similarly but expressed varyingly. SV2C, a minor isoform of SV2, expressed in a small subset of neurons located within the basal forebrain, midbrain and brainstem. SV2B, a major isoform of SV2 is expressed more abundantly, although rarely without the coexpression of SV2A. SV2A, the other major isoform of SV2 is the most widely expressed. SV2A is located in the presynaptic nerve terminals of almost every neuron throughout the nervous system. In addition, it is also located in most neuroendocrine secretory granules (1). SV2A has been identified as a critical protein for proper function of the central nervous system and has been linked to the physiopathology of epilepsy (2). In addition to the epileptic affects of this protein, mutations in it have also been seen to result in schizophrenia (3). Alternate Names KIAA0736; SV2; SV2A; synaptic vesicle glycoprotein 2; Synaptic vesicle glycoprotein 2A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80-100 Source/Isotype: Rabbit IgG