CST, 2894S, FGF Receptor 4 Antibody

Polyclonal Antibody for studying FGFR4. Validated for Western Blotting. Highly specific and rigorously validated in-house, FGF Receptor 4 Antibody (CST #2894) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FGF Receptor 4 Antibody detects endogenous levels of FGF receptor 4 protein. This antibody does not cross-react with other related proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe794 of human FGF receptor 4. Antibodies are purified by protein A and peptide affinity chromatography. Background Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components, such as Crk and PLCγ (4,5). Alternate Names CD334; FGFR-4; FGFR4; Fibroblast growth factor receptor 4; hydroxyaryl-protein kinase; JTK2; MGC20292; protein-tyrosine kinase; TKF; tyrosine kinase related to fibroblast growth factor receptor; tyrosylprotein kinase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 125 SOURCE: Rabbit