CST, 2896T, p18 INK4C (DCS118) Mouse Monoclonal Antibody

Monoclonal Antibody for studying INK4C, p18. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, p18 INK4C (DCS118) Mouse Monoclonal Antibody (CST #2896) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity The p18 INK4C (DCS118) Mouse Monoclonal Antibody detects endogenous levels of p18 INK4C protein. This antibody does not cross-react with other proteins of the INK4 family. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with purified recombinant human p18 INK4C protein. Background Cyclin-dependent kinases (CDKs) are activated in part by forming complexes with cyclins. For example, CDK4 and CDK6 associate with the D-type cyclins and phosphorylate the retinoblastoma protein. This phosphorylation is a necessary event for cells to enter S-phase (1). The inhibitors of CDK4 (INK4) family include p15 INK4B, p16 INK4A, p18 INK4C and p19 INK4D. p18 has been shown to function as a haploinsufficient tumor suppressor (2). All INK4 proteins are composed of 32 amino acid ankyrin motifs and selectively inhibit CDK4/6 activity. Mutational analyses of p18 implicate the third and the amino-terminal portion of the fourth ankyrin repeat in mediating binding to CDK4/6 (3). The interaction of INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression (4,5). Alternate Names CDK6 inhibitor p18; CDKN2C; CDKN6; CDN2C; cyclin dependent kinase inhibitor 2C; cyclin-dependent inhibitor; Cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; cyclin-dependent kinase 6 inhibitor p18; cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4); INK4C; p18; p18-INK4c; p18-INK6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Mouse IgG2a