CST, 29021T, Phospho-PLCbeta3 (Ser537) (D8K2R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PLCb3 (Ser537) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PLCbeta3 (Ser537) (D8K2R) Rabbit Monoclonal Antibody (CST #29021) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-PLCbeta3 (Ser537) (D8K2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PLCbeta3 only when phosphorylated at Ser537. This antibody cross-reacts with a murine protein of ~75 kDa whose identity was not determined. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser537of human PLCβ3 protein. Background Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3). Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1248 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8). Alternate Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3; FLJ37084; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-beta-3; phospholipase C beta 3; Phospholipase C-beta-3; phospholipase C, beta 3 (phosphatidylinositol-specific); PLC beta 3; PLC-beta-3; PLCB3; SMDCD Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG