CST, 2909T, Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit Monoclonal Antibody (CST #2909) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit Monoclonal Antibody detects endogenous levels of proteins containing the ATM/ATR substrate motif. This antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. This antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with synthetic phospho-ATM/ATR substrate peptides. Background Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG